Fellowship des Landes Salzburg

  • Vlasak, Reinhard (Projektleitung)
  • Pachler, Karin, (Projektleitung)
  • Mayr, Juliane, (Projektleitung)

    Projektdetails

    Beschreibung

    The main purpose of this project is to establish a system to generate recombinant influenza C viruses. This will allow specific genetic analysis of the viral genome. In particular, we want to investigate the function of the viral NS1 protein. It appears interesting to determine whether this protein has similar functions as the NS1 proteins from influenza A and B viruses in terms of downregulation of the cellular interferon response pathways.

    To elucidate the function of the influenza C virus NS1 protein, we will produce recombinant influenza C viruses harbouring deletions in the NS1 coding sequence.

    Then we will test whether or not these mutant viruses are able to replicate in cells with functional IFN signalling, and we will compare the IFN response of NS1-mutated and wildtype viruses.

    In the future, the reverse genetics system for the rescue of influenza C viruses can be used for different approaches to test the function of viral proteins via modifications of the viral genome. This may allow an assessment of the potential of modified influenza C viruses for use as vectors for gene transfer and as tumor-destroying “oncolytic' viruses.

    Generation of recombinant influenza C viruses
    Preparation of influenza C viruses with NS1 deletions
    Assays to study the function of the NS1 protein

    In this project, we want to develop a system to generate recombinant influenza C viruses entirely from plasmids. A plasmid vector has been constructed, which allowed cloning and bidirectional expression of all seven RNA segments of the viral genome. The seven different plasmids will be transfected into cells which support replication and assembly of influenza C viruses. Similar to methods described for the generation of recombinant influenza A viruses, RNAs will be transcribed from the plasmids and translated within the cells in a way that finally allows assembly and release of recombinant viruses. Once the procedure to generate viruses from plasmids is established, we want to study the function of the viral non-structural protein NS1 by reverse genetics.
    KurztitelFellowship des Landes Salzburg
    StatusAbgeschlossen
    Tatsächlicher Beginn/ -es Ende1/01/0831/12/09

    Systematik der Wissenschaftszweige 2012 (6-Steller)

    • 303034 Virologie
    • 304003 Gentechnik

    Systematik der Wissenschaftszweige 2012

    • 106 Biologie
    • 304 Medizinische Biotechnologie

    Schlagwörter

    • Influenza C virus
    • Interferon
    • Nonstructural protein N51
    • Recombinant virus

    Systematik der Wissenschaftszweige 2002

    • 3428 Virologie
    • 1433 Gentechnik, -technologie