Abstract
The ability of bone graft substitutes to promote new bone formation has been increasingly used in the medical field to repair skeletal defects or to replace missing bone in a broad range of applications in dentistry and orthopedics. A common way to assess such materials is via micro computed tomography (µ-CT), through the density information content provided by the absorption of X-rays. Information on the chemical composition of a material can be obtained via Raman spectroscopy. By investigating a bone sample from miniature pigs containing the bone graft substitute Bio Oss®, we pursued the target of assessing to what extent the density information gained by µ-CT imaging matches the chemical information content provided by Raman spectroscopic imaging. Raman images and Raman correlation maps of the investigated sample were used in order to generate a Raman based segmented image by means of an agglomerative, hierarchical cluster analysis. The resulting segments, showing chemically related areas, were subsequently compared with the µ-CT image by means of a one-way ANOVA. We found out that to a certain extent typical gray-level values (and the related histograms) in the µ-CT image can be reliably related to specific segments within the image resulting from the cluster analysis.
Titel in Übersetzung | A bone sample containing a bone graft substitute analyzed by correlating density information obtained by X-ray micro tomography with compositional information obtained by Raman microscopy |
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Originalsprache | Englisch |
Seiten (von - bis) | 3831-3853 |
Seitenumfang | 23 |
Fachzeitschrift | Materials |
Jahrgang | 8 |
Ausgabenummer | 7 |
DOIs | |
Publikationsstatus | Veröffentlicht - 1 Jan. 2015 |
Bibliographische Notiz
8 (7)Systematik der Wissenschaftszweige 2012
- 103 Physik, Astronomie
- 104 Chemie
- 106 Biologie
- 301 Medizinisch-theoretische Wissenschaften, Pharmazie
- 302 Klinische Medizin