Intra- and intermuscular variations of postmortem protein degradation for PMI estimation

In recent years, protein decomposition has become of increasing interest for the use in forensic estimation of the postmortem
interval (PMI). Especially skeletal muscle tissue has proven to be a prime target tissue, among other reasons, due to its large
abundance in the human body. In this regard, it is important to know whether there are any intra- and intermuscular differences in
the behavior of protein degradation. Thus, samples from different locations within several skeletal muscles as well as from
cardiac and smooth muscle tissue samples were collected from three autopsy cases with varying degree of decomposition.
Samples were analyzed by SDS-PAGE and Western blotting and compared for protein degradation patterns. Intramuscular
variations turned out to be minimal and without major influence for the use of the method. Observed intermuscular differences
provide possibilities for future improvement of the precision and temporal application range. The results of this study show the
strengths and current limitations of protein degradation-based PMI estimation and provide a deeper understanding of
intraindividual postmortem protein degradation processes.