A semi-automated hybrid HPLC-MS approach for in-depth characterization of intact non-covalent heterodimer glycoforms of gonadotropin biopharmaceuticals

F Di Marco, G Blümel, C Blöchl, M Wuhrer, CG Huber

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Article number341574
Number of pages12
JournalAnalytica chimica acta
Volume1274
DOIs
Publication statusPublished - 15 Sept 2023

Bibliographical note

Funding Information:
This work was supported by the Christian Doppler Society , Austria, the Austrian Federal Ministry for Digital and Economic Affairs , the National Foundation of Research, Technology, and Development , a Start-up Grant of the State of Salzburg . Moreover, grants of the Austrian Science Fund ( W1213 , FG 12 N ) financially supported this work.

Funding Information:
This work was supported by the Christian Doppler Society, Austria, the Austrian Federal Ministry for Digital and Economic Affairs, the National Foundation of Research, Technology, and Development, a Start-up Grant of the State of Salzburg. Moreover, grants of the Austrian Science Fund (W1213, FG 12 N) financially supported this work.The authors declare the following competing financial interests: Thermo Fisher Scientific and Novartis AG provided financial support for the Christian Doppler Laboratory for Innovative Tools for Biosimilar Characterization. The salary of Christian G. Huber was partly funded by the Christian Doppler Laboratory for Biosimilar Characterization. The authors declare no other competing financial interest.

Publisher Copyright:
© 2023 The Authors

Keywords

  • Biopharmaceutical characterization
  • Glycosylation
  • Gonadotropin
  • HPLC-MS
  • Hybrid-MS
  • Intact glycoforms
  • hCG
  • hFSH
  • Cricetinae
  • Cricetulus
  • Humans
  • Chorionic Gonadotropin/analysis
  • Follicle Stimulating Hormone, Human
  • Chromatography, High Pressure Liquid
  • Pregnancy
  • Tandem Mass Spectrometry
  • Animals
  • Biological Products
  • Female
  • CHO Cells

Fields of Science and Technology Classification 2012

  • 104 Chemistry
  • 106 Biology

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