Comprehensive analysis of alterations in the miRNome in response to photodynamic treatment

Doris Bach, Julia Fuereder, Michael Karbiener, Marcel Scheideler, Ress Anna Lena, Daniel Neureiter, Ralf Kemmerling, Otto Dietze, Markus Wiederstein, Frieder Berr, Kristjan Plätzer, Tobias Kiesslich, Martin Pichler

    Research output: Contribution to journalArticle

    Abstract

    Photodynamic therapy (PDT) is a local tumour treatment accepted for a number of indications. PDT operates via the cellular stress response through the production of reactive oxygen species and subsequent cellular damage, resulting in cell death. Although PDT-induced signalling and cytotoxicity mechanisms have been investigated, the effect of PDT on microRNA (miRNA) expression is largely unknown. Therefore, we conducted a comprehensive microarray-based analysis of the miRNome of human epidermoid carcinoma cells (A431) following in vitro photodynamic treatment using polyvinylpyrrolidone hypericin (PVPH) as a photosensitiser and nearly homogeneous apoptosis-inducing conditions. Using microarray analysis we found eight miRNAs to be significantly differentially expressed 5h post treatment compared with the baseline levels and three miRNAs with more than 2-fold differential expression that could be detected in 1 or 2 biological replicates. The verification of these results by quantitative RT-PCR including a detailed time-course revealed an up to 15-fold transient over-expression of miR-634, miR-1246, miR-1290 and miR-487b compared with the basal level. For these miRNAs, in silico mRNA target prediction yielded numerous target transcripts involved in the regulation of cell stress, apoptosis, cell adherence and proliferation. This study provides the first comprehensive miRNome analysis after PDT treatment and may help to develop novel miRNA-based therapeutic approaches to further increase the efficiency of PDT.

    Translated title of the contributionComprehensive analysis of alterations in the miRNome in response to photodynamic treatment
    Original languageEnglish
    Pages (from-to)74-81
    Number of pages8
    JournalJournal of Photochemistry and Photobiology B: Biology
    Volume120
    DOIs
    Publication statusPublished - 5 Mar 2013

    Bibliographical note

    Copyright © 2013 Elsevier B.V. All rights reserved.

    Fields of Science and Technology Classification 2012

    • 106 Biology
    • 301 Medical-Theoretical Sciences, Pharmacy

    Keywords

    • Cell Line, Tumor
    • Humans
    • MicroRNAs/genetics
    • Oligonucleotide Array Sequence Analysis
    • Photochemotherapy
    • Photosensitizing Agents/pharmacology
    • Povidone/pharmacology
    • Time Factors
    • Transcriptome/drug effects

    Cite this

    Bach, D., Fuereder, J., Karbiener, M., Scheideler, M., Lena, R. A., Neureiter, D., Kemmerling, R., Dietze, O., Wiederstein, M., Berr, F., Plätzer, K., Kiesslich, T., & Pichler, M. (2013). Comprehensive analysis of alterations in the miRNome in response to photodynamic treatment. Journal of Photochemistry and Photobiology B: Biology, 120, 74-81. https://doi.org/10.1016/j.jphotobiol.2013.01.012